Doctor of Biological Sciences, Academician of the RAS (Russian Academy of Sciences), the Head of two subdivisions at the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBC): Innovative Center “IBCH Industrial Park” and the Laboratory of Molecular Technologies.
Dr Lukyanov’s main scientific interest is analysis of the structure and function of eukaryotic genomes. He is the author ofmore than 140 scientific publications in Russian and foreign journals and also of more than 20 Russian and foreign patents. Dr Lukyanov’s works are widely cited in the scientific literature (more than 8,500 references, and a score of 40 in the Hirsch Index).
Dr Lukyanovwon the 2006 OvchinnikovPrize awarded by the RAS Presidium for prominent works in physicochemical biology and biotechnology. The prize was awarded for authorship of the work titled “Fluorescent proteins: finding, investigation and use in biotechnology”. He also won prizes awarded by Nauka international academic publisher for best publications in Nauka journalsin 1996, 1999 and 2004, as well as he is the winner of competitions held by Bioorganic Chemistry journal for the monograph of the year in 1996, 1997, 1999, 2002, 2003, 2004, and 2005. Dr Lukyanov holds awards in Russia’s “Outstanding Scientists and Talented Young Researchers” Programme, and had been awarded the “State Scientific Scholarship” grant.
Dr Lukyanov’s work has been focused on finding, investigation and use of fluorescent proteins, which are homologues of green fluorescent proteins (GFRs). In 1999 Lukyanov’s laboratory discovered new fluorescent proteins inAnthozoacoral polyps that fluoresce from blue-green to red. Discovery of fluorescent and coloured GFR-like proteins in coral polyps clarified the origin of various fluorescent and non-fluorescent coloursof coral reefs which has beenan unexplained phenomenon for many years. The discovery has enabled substantial advances in techniques for cell marking in living organisms. Firstly, multi-colour labelling can now be used to observe several interrelated processes in a cell simultaneously. Discovery of proteins that change fluorescent colour on aging made it possible to follow the time pattern of gene expression. Red and far-red fluorescent proteins have offered fundamentally new prospectsfor monitoring normal and pathological processes in the whole organism.
Dr Lukyanovhas led the engineering of new-generation fluorescent sensors for monitoring of changes in key parameters of a live cell. A particularly important development in this direction is the unique HyPer high-sensitive sensor to detect presence of hydrogen peroxide (an important regulator of many biological processes) in living cells. Use of the HyPer sensor has appreciably enriched understanding of the kinetic profile ofhow active formsof oxygen are generated during apoptosis and phagocytosis,when cells are exposed to growth-stimulating factors.
Researches in Lukyanov’s laboratory that led to discovery of fluorescent proteins in coral polyps, development of photoactivatable fluorescent-protein technology, and creation of a genetically encoded photosensitizerwere acclaimed by the Russian Academy of Sciences as the main achievements in physicochemical biology in 1999, 2003,and 2005, respectively.
In2011 a team of scientists headed by Dr Lukyanov received a large grant from the Russian Government,which was invested in establishment of a fluorescent bio-imaging laboratory at Nizhny Novgorod State Medical Academy. The laboratory is developing techniques for visualizingthe processes that occur in development of oncological diseases in live model organisms. Fluorescent proteins and biosensors based on them will be the key investigative tools in this project.